Interspecific Transplantation of Developing Tissues and Their - BioStor

ZOOLOGICAL SCIENCE 5: 497-499 (1988) ? 1988 Zoological Society of Japan [COMMUNICATION] Interspecific Transplantation of Developing Tissues and Their Subsequent Differentiation in Flies Pakkirisamy Sivasubramanian Department of Biology, University of New Brunswick, Fredericton, New Brunswick, E3B 6E1, Canada ABSTRACT ? Imaginal leg discs from the larvae of housefly, Musca domestica were cultured in the body cavity of the pupae of fleshfly, Sarcophaga bullata. The implanted discs were not rejected by the foreign species. On the contrary, they differentiated fully and completed metamorphosis in time according to their own develop- mental program. However, the tanning and hardening of the cuticle occurred along with that of the host after eclosion of the host fly. These developmental events are discussed in relation to the hormonal milieu of the host species. INTRODUCTION During the course of their development flies go through distinct stages such as larva and pupa before metamorphosing into adults. Several adult structures like the legs, wings, eyes, etc., differ- entiate in the pupal stage from groups of embryonic cells called imaginal discs. Simul- taneously, the central nervous system (CNS) too undergoes considerable reorganization and estab- lishes specific neural connections with the newly formed peripheral target tissues. A great deal of information is available with regard to the forma- tion of specific nerve connections between the CNS and target tissues of the same species such as crickets [1], fleshflies [2] and fruitflies [3]. Howev- er, very little is known whether this specificity is restricted within a species or it extends beyond species boundaries. To explore this aspect of neuron-target interaction, developing tissues of Accepted September 16, 1987 Received August 4, 1987 the housefly, Musca domestica, were cultured into the pupal body cavity of the fleshfly, Sarcophaga bullata, and this report describes the metamor- phosis of such transplanted imaginal discs. The differentiation of transplanted CNS of the housefly has been reported elsewhere [4]. MATERIALS AND METHODS The housefly, Musca domestica, and the fleshfly, Sarcophaga bullata, were cultured in the labora- tory under constant conditions of temperature (25?C) and photoperiod (16L:8D). The housefly larvae were raised in an artificial diet containing milkpowder, wheat bran and sawdust, while the fleshfly maggots were fed with fresh beef liver. Since the housefly is smaller in size with a shorter pupal life they were used as donors, while the larger fleshfly with longer pupal period served as hosts. The imaginal leg discs of mature 3rd instar larvae of Musca domestica were dissected in insect saline and implanted into freshly formed fleshfly prepupae. The transplantation method was based on the technique of Bhaskaran and Sivasubramanian [5] but slightly modified as de- scribed in Sivasubramanian and Nassel [2]. Of the total of 76 successful implants, 36 were recovered 6 days after the operation from the host pupa (total pupal period of donors) and 40 were recovered 12 days post operation after eclosion of the meta- morphosed host flies. The tissues were examined as whole mounts.

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ZOOLOGICAL SCIENCE 5: 497-499 (1988) ? 1988 Zoological Society of Japan [COMMUNICATION] Interspecific Transplantation of Developing Tissues and Their Subsequent Differentiation in Flies Pakkirisamy Sivasubramanian Department of Biology, University of New Brunswick, Fredericton, New Brunswick, E3B 6E1, Canada ABSTRACT ? Imaginal leg discs from the larvae of housefly, Musca domestica were cultured in the body cavity of the pupae of fleshfly, Sarcophaga bullata. The implanted discs were not rejected by the foreign species. On the contrary, they differentiated fully and completed metamorphosis in time according to their own develop- mental program. However, the tanning and hardening of the cuticle occurred along with that of the host after eclosion of the host fly. These developmental events are discussed in relation to the hormonal milieu of the host species. INTRODUCTION During the course of their development flies go through distinct stages such as larva and pupa before metamorphosing into adults. Several adult structures like the legs, wings, eyes, etc., differ- entiate in the pupal stage from groups of embryonic cells called imaginal discs. Simul- taneously, the central nervous system (CNS) too undergoes considerable reorganization and estab- lishes specific neural connections with the newly formed peripheral target tissues. A great deal of information is available with regard to the forma- tion of specific nerve connections between the CNS and target tissues of the same species such as crickets [1], fleshflies [2] and fruitflies [3]. Howev- er, very little is known whether this specificity is restricted within a species or it extends beyond species boundaries. To explore this aspect of neuron-target interaction, developing tissues of Accepted September 16, 1987 Received August 4, 1987 the housefly, Musca domestica, were cultured into the pupal body cavity of the fleshfly, Sarcophaga bullata, and this report describes the metamor- phosis of such transplanted imaginal discs. The differentiation of transplanted CNS of the housefly has been reported elsewhere [4]. MATERIALS AND METHODS The housefly, Musca domestica, and the fleshfly, Sarcophaga bullata, were cultured in the labora- tory under constant conditions of temperature (25?C) and photoperiod (16L:8D). The housefly larvae were raised in an artificial diet containing milkpowder, wheat bran and sawdust, while the fleshfly maggots were fed with fresh beef liver. Since the housefly is smaller in size with a shorter pupal life they were used as donors, while the larger fleshfly with longer pupal period served as hosts. The imaginal leg discs of mature 3rd instar larvae of Musca domestica were dissected in insect saline and implanted into freshly formed fleshfly prepupae. The transplantation method was based on the technique of Bhaskaran and Sivasubramanian [5] but slightly modified as de- scribed in Sivasubramanian and Nassel [2]. Of the total of 76 successful implants, 36 were recovered 6 days after the operation from the host pupa (total pupal period of donors) and 40 were recovered 12 days post operation after eclosion of the meta- morphosed host flies. The tissues were examined as whole mounts.

Interspecific Transplantation of Developing Tissues and Their Subsequent Differentiation in Flies : COMMUNICATION : Developmental Biology

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